ABSTRACT
Objective: To explore the impacts of temperature, endogenous hormone, and pollination methods on the seeds germination and emergence of Paris polyphylla var. yunnanensis and polygerm varieties, and establish the technical system to promote the germination of P. polyphylla var. yunnanensis seeds and provide scientific guidance for the cultivation of Paris L., To ease the bottleneck problem of current serious shortage of high quality source of seeding material and commercial herbal products of Paris L., and highest market price. Methods: Using wet sand cultured P. polyphylla var. yunnanensis seeds under different conditions and determined the rate of germination and the mean germination time, the cultivated seeds were planted in the field to investigate the rate of seedling emergence and the average time of emergence. Results: The higher germination rate and emergence rate were obtained at the temperature of 4-18℃; Gibberellin (GA3) treatment could promote the seed germination, and reduce the mean germination time, especially in 100 and 200 mg/L; The germination rate and emergence rate of artificial pollination were significantly higher than natural pollination (P < 0.05); There is no difference between P. polyphylla var. yunnanensis and polygerm varieties seeds. Conclusion: The peeled seeds are treated by 100 mg/L gibberellin, and cultured to sprout by wet sand at the temperature of 4-18℃, then sown in the field. It can shorten the seed emergence period one year and up to more than 80% of emergence rate.
ABSTRACT
Objective: To establish a quantitative analysis method of seven steroidal saponins in Paridis Rhizoma and its polygerm varieties from different regions in Yunnan province, to simultaneously determine the contents of seven steroidal saponins, to establish the fingerprint for the polygerm varieties in Gaoligong Mountain by UPLC-ELSD, and to evaluate the qualities. Methods: It was detected with an Acquity UPLC® BEH C18 (2.1 mm × 50 mm, 1.7 μm) column, and gradually diluted with acetonitrile-water at a flow rate of 0.2 mL/min, drift tube temperature of 55℃, nitrogen pressure 275.8 kPa, and gain 500 by UPLC-ELSD. Results: The 23 samples could be separated and analyzed of seven steroids within 23 min. All the indexes of the methodological investigation met the requirements. The results showed that the contents of saponins from 19 samples met the requirement in Chinese Pharmacopoeia 2015. The average content of four saponins was 1.42% and among them the content from the sample in Gaoligong Mounta was up to 1.660%. The similarity was lower than 0.9 in the samples of Gaoligong Mountain, and there were eight common peaks in the fingerprints, which were identified four characteristic peaks. The type and content of seven saponins in the samples could not be clearly classified by PCA analysis and system cluster analysis. Conclusion: The method is convenient, accurate, and suitable for the quantitative analysis of Paridis Rhizoma and polygerm varieties. There is no obvious difference between Paridis Rhizoma and polygerm varieties in chemical components and contents. Paridis Rhizoma (polygerm varieties) in Gaoligong Mountain is a variety worth popularization and in-depth researches.